bovine skin type 1 collagen  (Advanced Biomatrix Inc)

Journal: BMC Biotechnology

Article Title: Recombinant type I & III chimeric collagen significantly enhances the vitality of fibroblasts and decelerates the skin aging process

doi: 10.1186/s12896-025-01050-9

Figure Lengend Snippet: Recombinant collagen promotes cell proliferation, migration and adhesion. A ) cell viability of NIH-3T3 cells assessed by the CCK-8 assay, the control is untreated ( n = 5). B ) cell migration is presented as scratch images after the administration of recombinant collagen, the control collagen is type I collagen from bovine skin. C ) quantification of cell migration rates derived from the scratch assay images ( n = 5). D ) cell adhesion was assessed after the administration of recombinant collagen, the control is untreated, the control collagen is type I collagen from bovine skin ( n = 5). E ) quantification of cell adhesion derived from assay images. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control

Article Snippet: The plates were coated with 5 μg/mL type I collagen from bovine skin (HY-NP101, MedChemExpress, Monmouth, NJ, USA) or DuCol before the cells were seeded.

Techniques: Recombinant, Migration, CCK-8 Assay, Control, Derivative Assay, Wound Healing Assay

Journal: BMC Biotechnology

Article Title: Recombinant type I & III chimeric collagen significantly enhances the vitality of fibroblasts and decelerates the skin aging process

doi: 10.1186/s12896-025-01050-9

Figure Lengend Snippet: Recombinant DuCol promotes the expression of endogenous type I & III collagen in fibroblasts. A ) expression of type I collagen and type III collagen after the administration of DuCol, as detected via western blotting. B ) quantification of type I collagen and type III collagen via western blotting ( n = 3). Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control

Article Snippet: The plates were coated with 5 μg/mL type I collagen from bovine skin (HY-NP101, MedChemExpress, Monmouth, NJ, USA) or DuCol before the cells were seeded.

Techniques: Recombinant, Expressing, Western Blot, Control

Journal: Biomolecules

Article Title: Tumor-Colonizing E. coli Expressing Both Collagenase and Hyaluronidase Enhances Therapeutic Efficacy of Gemcitabine in Pancreatic Cancer Models

doi: 10.3390/biom14111458

Figure Lengend Snippet: In vitro degradation of collagens and hyaluronic acid by BL21-TAN. Hydrolysis reactions were performed using uninduced (U) or induced (I) BL21-TAN co-incubated with FITC-conjugated pig skin gelatin ( A ), bovine skin collagen type I ( B ), human placenta collagen type IV ( C ), or purified hyaluronic acid (HA) ( D ) in 50 mM Tris-HCl (pH 8.0) containing 10 mM CaCl 2 at 37 °C. The negative control includes the culture media (LB). Increases in fluorescence intensity signify the degradation of the FITC-conjugated target. Enzyme activity was measured by monitoring fluorescence (FITC) (ex: 495 nm, em: 519 nm). Data are expressed as mean ± error of the mean of three independent experiments. **** p < 0.0001, t -test.

Article Snippet: To measure collagenase activity, substrates consisting of bovine skin collagen type I, human placenta collagen type IV, and pig skin gelatin conjugated to FITC (Thermo Fisher Scientific) were used.

Techniques: In Vitro, Incubation, Purification, Negative Control, Fluorescence, Activity Assay